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Forgot your password? Mol Biotechnol , 60— Plant Physiol , — J Chromatogr , — Hellerstein MK, Neese RA: Mass isotopomer distribution analysis at eight years: theoretical, analytic, and experimental considerations. Am J Physiol , EE Anal Chem , — Download references. Adrian D. Hegeman and Jerry Cohen are recognized for excellent discussions and explanations about the UMN algorithm and the protein turnover calculations. We also acknowledge Thomas F. Additionally, the authors would like to thank Ryan Geraets and Brett Kollars for their technical assistance.

Correspondence to Fedora Sutton. Reprints and Permissions. Search all BMC articles Search. Abstract Background The length of time that a protein remains available to perform its function is significantly influenced by its turnover rate. Conclusion We describe a more rapid and non-radioactive method to measure free amino acid and protein turnover.

Background There are various methods used to quantify proteins from different plant species [ 1 ] including the single cellular green alga Chlamydomonas reinhardtii [ 2 , 3 ]. Results and discussion Effect on growth in 15 N-labeled medium Growth curves are excellent indicators of the response of cells to stressful conditions. Figure 1. Full size image.

Figure 2. Figure 3. Table 1 C.

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Figure 4. Figure 5.

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Figure 6. Figure 7. Amino acids analysis Amino acid purification, derivatization, GC-MS and data analysis were performed as described [ 18 ]. Protein turnover analysis From the MS data, isotopic distributions were generated representing the fully labeled old peptide , fully unlabeled spiked control.

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References 1. Google Scholar Additional information Competing interests The authors declare that they have no competing interest. Electronic supplementary material Additional file 1: List of peptides for each of the protein identified from the search of the Chlamydomonas database in order of confidence levels largest to smallest.

PDF KB. Additional file 2: List of proteins with a minimum of two unique peptides with percent confidence levels of 95 and greater.

Hydrolyzed vegetable protein

PDF 91 KB. Additional file 3: Table S1: Identification of unique peptides and percent coverage for accepted proteins. Additional file 4: List of peptides identified for each of the five proteins at various time points in the experiments. PDF 86 KB. Additional file 5: List of the peptides for the three plastid proteins. PDF 59 KB.

Amino Acid |

Rights and permissions Reprints and Permissions. Contact us Submission enquiries: Access here and click Contact Us General enquiries: info biomedcentral. Choosing proteins, peptides, and amino acids as the subject matter for the second volume was simple, given their preeminence in regulating biochemical processes and their importance to modern molecular biology. The SourceBook series was inspired by our difficulty in locating a suitable replacement for a depleted reagent in the midst of an urgent research project.

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Manufacturer warranty may not apply Learn more about Amazon Global Store. From the Back Cover Explosive growth in the number of biological reagents available for sale to the research community has significantly complicated the process of finding a particular chemical or locating a suitable replacement for one that is no longer sold.